![]() ![]() Researchers can now reliably measure relative changes in protein expression and abundance.īut with this shift towards quantitative understanding comes a movement by major journals, funding agencies, and professional scientific societies to uphold the highest standards of data integrity for publication. In recent years, however, improved western blot technology and technique have allowed an exciting quantitative element to emerge. In its early days, the assay delivered information in a strict binary of ‘yes’ or ‘no’ – protein present or absent. Western blotting is a powerful tool in the sense that it can tease out the needle – a single protein of interest – in a proverbial haystack sample containing hundreds of proteins.įor more than 40 years, the western blot or immunoblot has been a workhorse of the wet lab. More Resources for Western Blot Reporting Journal-Specific Western Blot Publication GuidelinesĪmerican Association of Cancer Research (AACR) Multiplexing can capture up to four proteins in a single blot for more meaningful and representative experiments, a must for western blot publication.įor today’s top journals, total protein normalization and high-quality images are the keys to western blot publication Fluorescence detection multiplexing achieves more data from your sample with iBright. ![]()
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